Journal: ACS Applied Polymer Materials

Article Title: Effects of Polymer Properties on Solid-State Shear Pulverization: Thermoplastic Processability and Nanofiller Dispersibility

doi: 10.1021/acsapm.2c01932

Figure Lengend Snippet: Changes in T deg with respect to the respective neat polymer for the series of polymer/2 vol % graphite nanocomposites compounded by SSSP with 1, 3, and 5 passes.

Article Snippet: Natural graphite was chosen as the model filler material for the compounding study; graphite exists as flakes of microns in size, and the stacked sp 2 -hybridized graphene layers can be exfoliated and dispersed to varying degrees with SSSP., Grade 230U natural flake graphite (average particle size = 20 μm, density = 2.2 g/cm 3 ) from Asbury Carbons was used as-received, without chemical, thermal, or expansion treatment.

Techniques: Polymer

Journal: Nucleic Acids Research

Article Title: Chemical mapping of cytosines enzymatically flipped out of the DNA helix

doi: 10.1093/nar/gkn200

Figure Lengend Snippet: CAA reactivity of cytosines in cognate (G C GC/GMGC) and mismatch (G C GC/GMCC) 25-mer DNA duplexes upon interaction with M.HhaI (or its Q237G mutant) and cofactor product SAH. Autoradiograph of degradation products of the 25-mer duplexes 5′-labeled on the target (upper) strand after separation by electrophoresis on a 15% sequencing gel. Lane 1, G + A nucleotide cleavage marker; lanes 2–7, reactions with 200 mM CAA for 45 min at 20°C, containing 300 nM protein, 280 nM DNA and 0.1 mM SAH as follows: lane 2, upper G C GC strand alone (treated at 37°C); lane 3, G C GC/GMGC; lane 4, G C GC/GMGC + M.HhaI, lane 5, G C GC/GMGC + M.HhaI + SAH; lane 6, G C GC/GMGC + M.HhaI(Q237G); lane 7, G C GC/GMGC +M.HhaI (Q237G) + SAH; lanes 8–11, CAA reactions containing 30 nM protein and 10 nM DNA as follows: lane 8, G C GC/GMGC; lane 9, G C GC/GMGC + M.HhaI; lane 10, G C GC/GMCC; lane 11, G C GC/GMCC + M.HhaI.

Article Snippet: Lanes 1 and 5, G + A nucleotide cleavage marker; lanes 2–4, reactions with 50 mM CAA for 50 min at 37°C, containing 10 units/µl M.AluI and 105 nM DNA as follows: lane 2, upper AG C T strand alone; lane 3, AG C T/AGCT; lane 4, AG C T/AGCT + M.AluI; lanes 6–8, reactions with 100 mM CAA for 50 min at 20°C, containing 0.4 units/µl M.SssI and 20 nM DNA as follows: lane 6, upper G C GC strand alone (treated at 37°C); lane 7, G C GC/GMGC; lane 8, G C GC/GMGC + M.SssI.

Techniques: Mutagenesis, Autoradiography, Labeling, Electrophoresis, Sequencing, Marker

Journal: Nucleic Acids Research

Article Title: Chemical mapping of cytosines enzymatically flipped out of the DNA helix

doi: 10.1093/nar/gkn200

Figure Lengend Snippet: CAA reactivity of cytosines in the 42-mer AG C T/AGCT duplex upon interaction with M.AluI (left panel) and in the 25-mer G C GC/GMGC duplex upon interaction with M.SssI (right panel). Lanes 1 and 5, G + A nucleotide cleavage marker; lanes 2–4, reactions with 50 mM CAA for 50 min at 37°C, containing 10 units/µl M.AluI and 105 nM DNA as follows: lane 2, upper AG C T strand alone; lane 3, AG C T/AGCT; lane 4, AG C T/AGCT + M.AluI; lanes 6–8, reactions with 100 mM CAA for 50 min at 20°C, containing 0.4 units/µl M.SssI and 20 nM DNA as follows: lane 6, upper G C GC strand alone (treated at 37°C); lane 7, G C GC/GMGC; lane 8, G C GC/GMGC + M.SssI.

Article Snippet: Lanes 1 and 5, G + A nucleotide cleavage marker; lanes 2–4, reactions with 50 mM CAA for 50 min at 37°C, containing 10 units/µl M.AluI and 105 nM DNA as follows: lane 2, upper AG C T strand alone; lane 3, AG C T/AGCT; lane 4, AG C T/AGCT + M.AluI; lanes 6–8, reactions with 100 mM CAA for 50 min at 20°C, containing 0.4 units/µl M.SssI and 20 nM DNA as follows: lane 6, upper G C GC strand alone (treated at 37°C); lane 7, G C GC/GMGC; lane 8, G C GC/GMGC + M.SssI.

Techniques: Marker

Journal: ACS Applied Materials & Interfaces

Article Title: Single or Vortex Ferroelectric and Ferromagnetic Domain Nanodot Array of Magnetoelectric BiFe 0.9 Co 0.1 O 3

doi: 10.1021/acsami.4c01232

Figure Lengend Snippet: MFM observations of single BFCO nanodots. (a) AFM topographic image of an oxalic acid AAO nanodot marked with the blue circle in Figure c measured with an SSS-MFMR cantilever. (b) MFM image. The dashed line indicates the outward form of the dots. (c, d) Schematic illustrations of expected magnetic flux and contrast in MFM image for comparing magnetic monodomain and multiple domains. (e, f) AFM topographic and MFM image of oxalic acid AAO nanodots. The dashed line indicates the outward form of the dots. (g, h) Schematic MFM contrast explaining the magnetoelectic coupling in the topological vortex domain.

Article Snippet: The ferroelectric and magnetic domains were observed by PFM and MFM with the Cypher S. The measurement conditions were the same as in our previous paper, but we changed the magnetically coated tip to a sharper one (Nanosensors SSS-MFMR: magnetic resolution <25 nm) to obtain high-resolution MFM images.

Techniques: